The real-time fluorescent quantitative PCR detector is a kind of instrument that can label and track the PCR products through fluorescent dyes or fluorescent labeled specific probes, and monitor the reaction process in real time. Combined with the corresponding software, the qualitative and quantitative analysis of the products can be carried out, and the initial concentration of the sample template to be measured can be calculated Finally, the unknown templates were quantitatively analyzed by standard curve, including TaqMan fluorescent probe and SYBR fluorescent dye. PCR can be used to quantify the abundance of specific genes or species, and compare the differences between different treatments or samples, such as gene expression level, to study the response of gene expression to the treatment, At the same time, PCR amplification efficiency and other information can be obtained.
The rapid real-time quantitative PCR instrument is an integrated platform for specific target gene detection and quantification. The rapid real-time quantitative PCR instrument combines the PCR thermal cycle, fluorescence detection and various application analysis software, and can dynamically observe the gradual increase of PCR amplification products in each reaction tube in each cycle of PCR. Quantitative results can be obtained immediately after the PCR experiment, No gel electrophoresis analysis is needed, no PCR products need to be purified, no experimental operation is necessary.
|Net Weight||11 Kg|
|Operation system||win7 system or above|
|indicator light||Standby, yellow and green switching indication of temperature rising and falling|
|COM Interface||Square USB|
Two. Product highlights
The btk-48 / 96 real-time fluorescence quantitative PCR system uses 96 well reaction plate or single and 8-linked plates, and the reaction volume is 5-120 μ L. the experiment can be completed in less than 2 hours.
Btk-48 / 96 real-time fluorescence quantitative PCR system has the advantages of saving time, high sensitivity, good accuracy and wide linear range.
Btk-48 / 96 real-time fluorescence is the whole 96 sample faces of quantitative PCR system. The same halogen pigeon lamp is used as the light source, and the halogen magpie light is used to excite each sample crystal after passing through 4 ~ 6 color light source filter. The excitation effect of long wavelength red fluorescence label is improved by adding light source filter to halogen ridge lamp light source, which can greatly improve the sensitivity of red fluorescence detection and the accuracy of quantitative analysis.
The excited fluorescence is then passed through a polychromatic fluorescent filter to the CCD camera to detect the signal ™ /SYBR® Green I,VIC®/JOE,NED Gamma /AMRA Gamma / Cy3®, ROX Gamma /A variety of fluorescent dyes including Texas Red and Cy5.
The core components of btk-48 / 96 real-time fluorescence quantitative PCR system include halogen magpie lamp light source, cold state CCD camera and semiconductor temperature control module. Imported components are used to fully ensure the sensitivity, accuracy, uniformity and repeatability of the instrument.
Three. After-sales service:
Our nucleic acid R&D laboratory team has more than ten years of experience in the industry and provides powerful after-sales support.
System operation interface